Sybr Green Master Mix Thermo

Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region.

Sybr green master mix thermo. Powertrack sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr. Thermo scientific maxima sybr green qpcr master mix is a ready to use solution optimized for qpcr and 2 step rt qpcr. Only template and primers need to be added. Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis.

Sybr green pcr master mix in a 1 ml vial. Sybr select master mix is a superior and cost effective solution for your real time pcr applications. Direct detection of pcr product is monitored by measuring the increase in fluorescence caused by the binding of sybr green dye to double stranded ds dna. Powerup sybr green master mix can be used in either standard or fast cycling mode and is compatible with all applied biosystems real time pcr instruments.

The sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. Power sybr green pcr master mix provides greater flexibility reduces experimental design time and requires fewer reagents. The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr. Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows.

Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa. The master mixes include maxima hot start taq dna polymerase and dntps in an optimized pcr buffer. Powerup sybr green master mix features include. For each master mix to be tested prepare enough master mix for 26 reactions 20 µl each according.

Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume. 2 it is also compatible with the bio rad iq 5 and cfx systems roche lightcycler 480 and agilent mx3005p systems. Amount shown is for a final primer concentration of 500 nm. Add 5 µl of yellow sample buffer to the 10 6 dilution and 4 5 µl to all of the other dilutions.

The recommended primer concentration range for powertrack sybr green master mix is 300 800 nm. Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. For optimal results recommended primer concentrations should be between 300 800 nm. Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr.